Protein purification with nanoparticle-enhanced crystallisation

Novel Nanoparticle Formulations with Enhanced F Relaxation

Introduction Fluorine MRI is receiving increasing attention, primarily due to the high NMR sensitivity of F nuclei, the physiological absence of fluorine containing substances and the availability of contrast materials based on bio-compatible perfluorinated carbon (PFC) nanostructures. The applicability of F MR as a molecular and cellular imaging technique has been demonstrated in a number of s...

Crystallisation-enhanced bulk hole mobility in phenothiazine-based organic semiconductors

A series of three novel donor-acceptor systems based on C(3)-malononitrile-substituted phenothiazines was synthesised in good overall yields and their thermal, spectroscopic, and electrochemical properties were characterised. The compounds were prepared through a sequence of Ullmann-coupling, Vilsmeier-Haack formylation and Knoevenagel-condensation, followed by Suzuki-coupling reactions for int...

Nanoparticle enhanced imaging.

Nanotechnology refers to the research and technical development of objects in a scale range of 1 to 100 nanometers. Particles that are nano-sized are opening new research avenues in the field of engineering and life sciences. Imaging with biocompatible nanoparticles has emerged as one of the most promising new diagnostic imaging technology fulfilling the goal of developing cost-effective magnet...

Simplified, Enhanced Protein Purification Using an Inducible, Autoprocessing Enzyme Tag

We introduce a new method for purifying recombinant proteins expressed in bacteria using a highly specific, inducible, self-cleaving protease tag. This tag is comprised of the Vibrio cholerae MARTX toxin cysteine protease domain (CPD), an autoprocessing enzyme that cleaves exclusively after a leucine residue within the target protein-CPD junction. Importantly, V. cholerae CPD is specifically ac...

Protein purification from whole tissues . Fatemeh Mehrpooyan*